Aperçu rapide pour Âne anti-Lapin IgG (Fc Region) Anticorps (ABIN102058)
Antigène
IgG
Clonalité
Polyclonal
Application
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
Isotype
IgG
Épitope
Fc Region
Reactivité
Lapin
Hôte
Âne
N° du produit (Fournisseur)
211-7103
Fournisseur
Rockland
Fonction
Rabbit IgG Fc Antibody
Réactivité croisée (Details)
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Rabbit IgG, Rabbit IgG F(c) and Rabbit Serum. No reaction was observed against Rabbit IgG F(ab).
Attributs du produit
Anti-Rabbit IgG (H&L) Fluorescein Antibody generated in mouse detects reactivity to Rabbit IgG.
Purification
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Application Note: Anti-Rabbit IgG F(c) antibody is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 5.0 mL
Buffer
Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, Preservative:0.01 % (w/v) Sodium Azide
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C,-20 °C
Stockage commentaire
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Date de péremption
12 months
Antigène
IgG
Classe de substances
Antibody
Sujet
Receptors bind the Fc portion of rabbit IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.